70 Animes of Tsunade entrusted with the autopsy and examination to Shizune, Sakura and Ino while Shikamaru, Kakashi and Naruto decipher the clues and the meaning of the message sent by Jiraiya.
Upon learning that the Raikage Sasuke Uchiha is the leader of the team that was responsible for the capture of his brother, sent a messenger to tell Tsunade. Shortly thereafter, Pain along with their bodies and Konan begin the invasion of Konoha. While Shizune discovers that the metals that exist in all bodies, transferring chakra begins the destruction of Konoha so they will tell you as soon as Tsunade, which looks out his window all the smoke and fire coming out of the village thus recalling the prophecy of the Sanin and sending call Naruto.
Then, counselors enter the Hokage's room and they criticize his decision to call Naruto because, for them is still a child and that if it falls into the hands of Akatsuki Kyubi would. Tsunade explains that Naruto is no longer a child and as a Konoha Shinobi to protect his village, and directs the connection frog Fukasaku alerted to the problem. Later all leave the room, and Danzo killed the frog.
Later she calls Katsuyu, and orders him to remove some of your chakra and heal all the ninjas. Meanwhile, Tsunade free chakra healing that strengthens the skills of Katsuyu.
At that moment, with its radar-jutsu, is the sector where Kakashi Hut and have been hurt and angry for not knowing the exact state of Kakashi, then, Choji arrives and tells all Pain information and says he has died and Kakashi Hut too, but the Fifth sent in search of Choza since it is alive. Finally, Pain reaches where Tsunade, and this reminds him of the threat of Pain, Tsunade saying that this is the last of the three legendary sannin.
At the same moment, Pain explains Akatsuki's intentions and asks where is Naruto, she refuses to answer and she says she does not underestimate her or Naruto. Later, when Yahiko Pain "finds the location of Naruto she is surprised.
At that moment, collects chakra in his feet to fight with Yahiko, but this makes the Shinra Tensei.
Then she saw that Shinra Pain performed using the Ninpo Tensei: Souzou Saisei, to protect and villagers of Konoha ninjas, saving everyone and is ready to fight Pain, even when exhausted. Pain attacks her with the body of the demons, but Naruto is involved destroying the body easily later orders Gamakichi Naruto Tsunade withdraw from the fight, and tells Godaime to carry Katsuyu. At that moment he meets Sakura (Tsunade) loses its youthful appearance and not have chakra. Later is in a coma for the excessive use of chakra, so the council decided to remove from office and put in your lugar.Pero Danzou recently in the manga 488, Tsunade awakens from coma, just before Kakashi is designated as the new Hokage.
